home
***
CD-ROM
|
disk
|
FTP
|
other
***
search
/
Shareware Overload Trio 2
/
Shareware Overload Trio Volume 2 (Chestnut CD-ROM).ISO
/
dir26
/
med9408c.zip
/
M9480418.TXT
< prev
next >
Wrap
Text File
|
1994-08-20
|
2KB
|
38 lines
Document 0418
DOCN M9480418
TI IL-10 inhibits HIV-1 replication and is induced by tat.
DT 9410
AU Masood R; Lunardi-Iskandar Y; Moudgil T; Zhang Y; Law RE; Huang CL; Puri
RK; Levine AM; Gill PS; Department of Medicine, University of Southern
California, Los; Angeles 90033.
SO Biochem Biophys Res Commun. 1994 Jul 15;202(1):374-83. Unique Identifier
: AIDSLINE MED/94311907
AB Interleukin 10 (IL-10) is produced by TH2 lymphocytes and regulates both
lymphoid and myeloid cells. In the present study we demonstrate that
IL-10 is expressed and produced spontaneously in the peripheral blood
mononuclear cells (PBMCs) of all HIV-1 infected individuals tested, 3 of
19 cases of HIV-negative lymphoma and none of five healthy controls.
IL-10 mRNA was detectable in both monocytes/macrophages and T
lymphocytes isolated from PBMCs of HIV infected patients. We have also
shown that infection of promonocytic (U937) and T (H9) cell lines with
HIV stimulates IL-10 secretion. Furthermore, a T cell line (H9) stably
transfected with a HIV tat expression-vector secreted higher levels of
IL-10. We have also demonstrated that rhIL-10 inhibited HIV-1
replication in infected monocytes and PBMCs in a dose dependent manner.
IL-10 may thus participate in long latency between HIV-1 infection and
development of AIDS.
DE Acquired Immunodeficiency Syndrome/BLOOD/*IMMUNOLOGY
B-Lymphocytes/*IMMUNOLOGY/METABOLISM Base Sequence Cell Line DNA
Primers Gene Expression Gene Products, tat/*METABOLISM Genes, tat
Human HIV Seronegativity HIV-1/DRUG EFFECTS/*PHYSIOLOGY
Interleukin-10/BIOSYNTHESIS/*PHARMACOLOGY/*PHYSIOLOGY
Lymphoma/BLOOD/IMMUNOLOGY Macrophages/IMMUNOLOGY Molecular Sequence
Data Monocytes/IMMUNOLOGY/METABOLISM Polymerase Chain Reaction/METHODS
Reference Values RNA, Messenger/ANALYSIS/BIOSYNTHESIS Support, U.S.
Gov't, P.H.S. T-Lymphocytes/*IMMUNOLOGY/METABOLISM Transfection Virus
Replication/DRUG EFFECTS/*PHYSIOLOGY JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).